DNA hybridization to mismatched templates: A chip study

DNA hybridization to mismatched templates: a chip study.

High-density oligonucleotide arrays are among the most rapidly expanding technologies in biology today. In the GeneChip system, the reconstruction of the sample mRNA concentrations depends upon the differential signal generated by hybridizing the RNA to two nearly identical templates: a perfect match probe (PM) containing the exact biological sequence; and a single mismatch (MM) differing from ...

Numerical modeling of DNA-chip hybridization with chaotic advection.

We present numerical simulations of DNA-chip hybridization, both in the "static" and "dynamical" cases. In the static case, transport of free targets is limited by molecular diffusion; in the dynamical case, an efficient mixing is achieved by chaotic advection, with a periodic protocol using pumps in a rectangular chamber. This protocol has been shown to achieve rapid and homogeneous mixing. We...

Electrokinetically controlled DNA hybridization microfluidic chip enabling rapid target analysis.

Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface nucleic acid hybridization, such as microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. One of the drawbacks of this reaction is the relativel...

Towards better DNA chip hybridization using chaotic advection

K-ras mutation detection by hybridization to a polypyrrole DNA chip.

BACKGROUND Detection of mutations in cancer-related genes is of major importance for both basic knowledge and clinical practice. Several strategies have been developed to diagnose these alterations. We describe a method based on polypyrrole DNA chip technology to detect K-ras gene mutations in tumors. METHODS An oligodeoxynucleotide array was constructed on a silicon device by copolymerizatio...